Journal article
Comprehensive lipidome profiling of isogenic primary and metastatic colon adenocarcinoma cell lines
CJ Fhaner, S Liu, H Ji, RJ Simpson, GE Reid
Analytical Chemistry | Published : 2012
DOI: 10.1021/ac302154g
Abstract
A "shotgun" lipidomics strategy consisting of sequential functional group selective chemical modification reactions coupled with high-resolution/accurate mass spectrometry and "targeted" tandem mass spectrometry (MS/MS) analysis has been developed and applied toward the comprehensive identification, characterization and quantitative analysis of changes in relative abundances of >600 individual glycerophospholipid, glycerolipid, sphingolipid and sterol lipids between a primary colorectal cancer (CRC) cell line, SW480, and its isogenic lymph node metastasized derivative, SW620. Selective chemical derivatization of glycerophosphoethanolamine and glycerophosphoserine lipids using a "fixed charge..
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Awarded by National Institute of General Medical Sciences
Funding Acknowledgements
Support for this work was provided by funding from the National Institutes of Health (Grant GM103508) to G.E.R. and, in part, from the National Health and Medical Research Council of Australia (Program Grant No. 487922) to R.J.S. and H.J.